Narishige Pc10 Manual New

Ensure the main power switch is and the unit is unplugged.

Adjust the dial to soften the glass without melting it completely during the first drop.

The story of the is one of a legendary laboratory workhorse that served as the global standard for micropipette fabrication for decades before its retirement. Known for its "gravity-fall" vertical pulling method, it allowed researchers to create ultra-fine needles for microinjection and patch-clamp experiments with remarkable consistency. The Legacy of the PC-10 narishige pc10 manual new

One such hero—a retired engineer in Osaka—recently uploaded a 24-page "restoration manual" that includes:

The Narishige PC-10 is a classic, dual-stage vertical micropipette puller highly regarded in electrophysiology, microinjection, and cellular biology laboratories worldwide. It utilizes gravity and a multi-stage heating element to draw glass capillaries into consistent, high-quality micropipettes or microinjection needles. Ensure the main power switch is and the unit is unplugged

Determines the tip size and sharpness. 4. Step-by-Step Operating Procedures

Narishige International (Narishige Group) still supports legacy products. While the PC-10 is discontinued, they often retain PDF archives. Known for its "gravity-fall" vertical pulling method, it

Understanding the anatomy of the PC-10 ensures precise adjustments and accurate troubleshooting. Core Component Checklist

Rotate the bottom switch 90 degrees counterclockwise to the "under weight" position (if applicable to your version). Press the red Start Switch

| Heat setting | Weight | Resulting pipette resistance (3 mm tip) | |--------------|--------|------------------------------------------| | 55 | 150g | No separation – too cold | | 62 | 150g | 2–3 MΩ (good for whole-cell patch) | | 68 | 150g | 5–7 MΩ (sharp intracellular) | | 75 | 200g | 10–12 MΩ (too long taper, fragile) | | 80+ | 100g | Blunt pipette – overheated glass |

To tailor this guide for your specific laboratory application, please share a few more details: